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KMID : 0881720140290040347
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Journal of Food Hygiene and Safety
2014 Volume.29 No. 4 p.347 ~ p.355
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Development of Species-Specific PCR to Determine the Animal Raw Material
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Kim Kyu-Heon
Lee Ho-Yeon
Kim Young-Sang
Kim Mi-Ra
Jung Yoo-Kyung
Lee Jae-Hwang
Jang Hye-Sook
Park Yong-Choon
Kim Sang-Yub
Choi Jang-Duck
Jang Young-Mi
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Abstract
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In this study, the detection method was developed using molecular biological technique to distinguish authenticity of animal raw materials. The genes for distinction of species about animals targeted at Cytochrome c oxidase subunit I (COI), Cytochrome b (Cytb), and 16S ribosomal RNA (16S rRNA) genes in mitochondrial DNA.The species-specific primers were designed by that Polymerase Chain Reaction (PCR) product size was around 200bp for applying to processed products. The target 24 raw materials were 2 species of domestic animals, 6 species of poultry, 2 species of freshwater fishes, 13 species of marine fishes and 1 species of crustaceans. The results of PCR for Rabbit, Fox, Pheasant, Domestic Pigeon, Rufous Turtle Dove, Quail, Tree Sparrow, Barn Swallow, Catfish, Mandarin Fish, Flying Fish, Mallotus villosus, Pacific Herring, Sand Lance, Japanese Anchovy, Small Yellow Croaker, Halibut, Jacopever, Skate Ray, Ray, File Fish, Sea Bass, Sea Urchin, and Lobster raw materials were confirmed 113 bp ~ 218 bp, respectively. Also, non-specific PCR products were not detected in compare species by species-specific primers. The method using primers developed in this study may be applied to distinguish an authenticity of food materials included animal raw materials for various processed products.
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KEYWORD
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Animal raw material, Polymerase Chain Reaction (PCR), Species-specific primer, mitochondrial DNA
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